Molecular cell, 2013-11, Vol.52 (4), p.583-590
2013
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- Autor(en) / Beteiligte
- Titel
- R loops are linked to histone H3 S10 phosphorylation and chromatin condensation
- Ist Teil von
- Molecular cell, 2013-11, Vol.52 (4), p.583-590
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- R loops are transcription byproducts that constitute a threat to genome integrity. Here we show that R loops are tightly linked to histone H3 S10 phosphorylation (H3S10P), a mark of chromatin condensation. Chromatin immunoprecipitation (ChIP)-on-chip (ChIP-chip) analyses reveal H3S10P accumulation at centromeres, pericentromeric chromatin, and a large number of active open reading frames (ORFs) in R-loop-accumulating yeast cells, better observed in G1. Histone H3S10 plays a key role in maintaining genome stability, as scored by ectopic recombination and plasmid loss, Rad52 foci, and Rad53 checkpoint activation. H3S10P coincides with the presence of DNA-RNA hybrids, is suppressed by ribonuclease H overexpression, and causes reduced accessibility of restriction endonucleases, implying a tight connection between R loops, H3S10P, and chromatin compaction. Such histone modifications were also observed in R-loop-accumulating Caenorhabditis elegans and HeLa cells. We therefore provide a role of RNA in chromatin structure essential to understand how R loops modulate genome dynamics.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/j.molcel.2013.10.006Titel-ID: cdi_proquest_miscellaneous_1544009376
- Format
- –
- Schlagworte
- Animals, Caenorhabditis elegans, Caenorhabditis elegans - genetics, Caenorhabditis elegans Proteins - metabolism, Chromatin Assembly and Disassembly, Chromatin Immunoprecipitation, DNA, Single-Stranded - genetics, Genomic Instability, HeLa Cells, Histones - metabolism, Humans, Meiosis, Mitosis, Open Reading Frames, Phosphorylation, Protein Processing, Post-Translational, RNA Polymerase II - metabolism, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae Proteins - metabolism, Transcription, Genetic