Chemical communications (Cambridge, England), 2014-05, Vol.50 (40), p.5313-5315
2014
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Details
- Autor(en) / Beteiligte
- Titel
- Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe
- Ist Teil von
- Chemical communications (Cambridge, England), 2014-05, Vol.50 (40), p.5313-5315
- Ort / Verlag
- England
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1359-7345eISSN: 1364-548XDOI: 10.1039/c3cc47444eTitel-ID: cdi_proquest_miscellaneous_1541412701
- Format
- –
- Schlagworte
- Assaying, Biological Assay, Biosensing Techniques, Coordination Complexes - chemistry, DNA Probes - chemistry, DNA, Single-Stranded - chemistry, G-Quadruplexes, Humans, Inhibitors, Iridium - chemistry, Kinases, Luminescence, Luminescent Measurements, Phosphorylation, Polynucleotide 5'-Hydroxyl-Kinase - metabolism, Polynucleotides, Screening