Details

Autor(en) / Beteiligte

• Liu, Cheng-xi
• Wu, Fan-lin
• Jiang, He-wei
• He, Xiang
• Guo, Shu-juan
• Tao, Sheng-ce

Titel

Global identification of CobB interactors by an Escherichia coli proteome microarray

Ist Teil von

• Acta biochimica et biophysica Sinica, 2014-07, Vol.46 (7), p.548-555

Ort / Verlag

China

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Protein acetylation is one of the most abundant post translational modifications and plays critical roles in many important biological processes. Based on the recent advances in mass spectrometry technology, in bacteria, such as Escherichia coli, tremendous acetylated proteins and acetylation sites have been identified. However, only one protein deacetylase, i.e. CobB, has been identified in E. coli so far. How CobB is regulated is still elusive. One right strategy to study the regulation of CobB is to globally identify its interacting proteins. In this study, we used a proteome microarray containing 4000 affinitypurified E. coli proteins to globally identify CobB interactors, and finally identified 183 binding proteins of high stringency. Bioinformatics analysis showed that these interacting pro teins play a variety of roles in a wide range of cellular func tions and are highly enriched in carboxylic acid metabolic process and hexose catabolic process, and also enriched in transferase and hydrolase. We further used biolayer inter ferometry to analyze the interaction and quantify the kinetic parameters of putative CobB interactors, and clearly showed that Cobb could strongly interact with TopA and AccC. The novel CobB interactors that we identified could serve as a start point for further functional analysis.