Details

Autor(en) / Beteiligte

• Lin, Xu-Hong
• Wei, Dan-Dan
• Wang, Hui-Chao
• Wang, Bin
• Bai, Chun-Yang
• Wang, Ya-Qiang
• Li, Guo-En
• Li, Hui-Ping
• Ren, Xue-Qun

Titel

Role of orphan G protein-coupled receptor 55 in diabetic gastroparesis in mice

Ist Teil von

• Sheng li hsüeh pao, 2014-06, Vol.66 (3), p.332-340

Ort / Verlag

China

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The aim of the present study was to explore the role of orphan G protein-coupled receptor 55 (GPR55) in diabetic gastroparesis (DG). Streptozotocin (STZ) was used to mimic the DG model, and the body weight and blood glucose concentration were tested 4 weeks after STZ injection (i.p.). Electrogastrogram and phenolsulfonphthalein test were used for detecting gastric emptying. Motilin (MTL), gastrin (GAS), vasoactive intestinal peptide (VIP), and somatostatin (SS) levels in plasma were determined using radioimmunology. Real-time PCR and Western blot were applied to identify the expression of GPR55 in gastric tissue, and immunohistochemistry was used to detect the distribution. The effect of lysophosphatidylinositol (LPI), an agonist of GPR55, was observed. STZ mice showed increased blood glucose concentration, lower body weight, decreased amplitude of slow wave, and delayed gastric emptying. LPI antagonized these effects of STZ. Compared to the control group, STZ caused significant decreases of MTL and GAS level