Plant physiology and biochemistry, 2013-12, Vol.73, p.93-98
2013
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- Autor(en) / Beteiligte
- Titel
- Increasing the starch content and grain weight of common wheat by overexpression of the cytosolic AGPase large subunit gene
- Ist Teil von
- Plant physiology and biochemistry, 2013-12, Vol.73, p.93-98
- Ort / Verlag
- Paris: Elsevier Masson SAS
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- ADP-glucose pyrophosphorylase (AGPase) catalyzes the first committed step of starch synthesis. AGPase is a heterotetramer composed of two large subunits and two small subunits, has cytosolic and plastidial isoforms, and is detected mainly in the cytosol of endosperm in cereal crops. To investigate the effects of AGPase cytosolic large subunit gene (LSU I) on starch biosynthesis in higher plant, in this study, a TaLSU I gene from wheat was overexpressed under the control of an endosperm-specific promoter in a wheat cultivar (Yumai 34). PCR, Southern blot, and real-time RT-PCR analyses indicated that the transgene was integrated into the genome of transgenic plants and was overexpressed in their progeny. The overexpression of the TaLSU I gene remarkably enhanced AGPase activity, endosperm starch weight, grain number per spike, and single grain weight. Therefore, we conclude that overexpression of the TaLSU I gene enhances the starch biosynthesis in endosperm of wheat grains, having potential applications in wheat breeding to develop a high-yield wheat cultivar with high starch weight and kernel weight. •TaLSU I was overexpressed in grains of common wheat.•Overexpression of TaLSU I increased activity of AGPase enzyme and contents of starch.•TaLSU I gene enhanced grain weights.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0981-9428eISSN: 1873-2690DOI: 10.1016/j.plaphy.2013.09.003Titel-ID: cdi_proquest_miscellaneous_1540231116
- Format
- –
- Schlagworte
- AGPase, Biological and medical sciences, biosynthesis, breeding, cultivars, cytosol, Cytosol - enzymology, Cytosol - metabolism, Cytosolic large subunit, endosperm, Endosperm - enzymology, Endosperm - growth & development, Endosperm - metabolism, Fundamental and applied biological sciences. Psychology, gene overexpression, Genes, Plant, Glucose-1-Phosphate Adenylyltransferase - genetics, Glucose-1-Phosphate Adenylyltransferase - metabolism, grain crops, Overexpression, Plant physiology and development, Plant Proteins - genetics, Plant Proteins - metabolism, Plants, Genetically Modified, progeny, Protein Subunits - genetics, Protein Subunits - metabolism, reverse transcriptase polymerase chain reaction, seeds, Southern blotting, Starch, Starch - biosynthesis, Starch - genetics, transgenes, transgenic plants, Triticum - enzymology, Triticum - genetics, Triticum - growth & development, Triticum - metabolism, Triticum aestivum, wheat, Wheat (Triticum aextivum L.)