Oncogene, 2014-06, Vol.33 (23), p.2978-2986
2014
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- Autor(en) / Beteiligte
- Titel
- The male-specific factor Sry harbors an oncogenic function
- Ist Teil von
- Oncogene, 2014-06, Vol.33 (23), p.2978-2986
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Sgf29, a component of the SPT-ADA-GCN5 acetyltransferase (SAGA) complex, binds H3K4me2/3 marks and leads to histone H3 acetylation. Previously, we found that downregulation of Sgf29 suppresses c-Myc-mediated malignant transformation. Nonetheless, the upstream regulator of the Sgf29 gene is not yet known. Here, we report that Sry (sex-determining region Y), an HMG (high-mobility group) domain containing transcription factor, directly upregulates Sgf29 gene expression. Sry expression was deregulated in two out of the four tested male rodent hepatocellular carcinoma (rHCC) cell lines. Luciferase reporter and chromatin immunoprecipitation assays indicated that Sry could bind HMG-boxes in the proximal promoter region of the Sgf29 gene. Knockdown of Sry robustly lowered anchorage-independent growth, invasiveness and tumorigenicity of rHCC cells, whereas ectopic expression of Sry conferred more malignant properties. Thus, these data show that Sry is involved in male-specific malignant conversion of rHCCs via Sgf29 upregulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-9232eISSN: 1476-5594DOI: 10.1038/onc.2013.262Titel-ID: cdi_proquest_miscellaneous_1540219279
- Format
- –
- Schlagworte
- 631/67/1504/1610, 692/420/755, Acetylation, Acetyltransferase, Acetyltransferases - genetics, Acetyltransferases - metabolism, Animals, Apoptosis, c-Myc protein, Cancer, Carcinogenesis, Carcinoma, Hepatocellular - genetics, Carcinoma, Hepatocellular - metabolism, Causes of, Cell Biology, Cell Line, Cell Line, Tumor, Cells, Chromatin, Ectopic expression, Gene expression, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, Genetic aspects, Health aspects, Hepatocellular carcinoma, Hepatoma, Histone Acetyltransferases - genetics, Histone Acetyltransferases - metabolism, Histone H3, Histones, HMG-Box Domains, Human Genetics, Immunology, Immunoprecipitation, Internal Medicine, Invasiveness, Liver cancer, Male, Males, Medicine, Medicine & Public Health, Mice, Mice, SCID, Myc protein, Neoplasm Invasiveness, Oncogenes, Oncology, Oncology, Experimental, original-article, Promoter Regions, Genetic, Protein Structure, Tertiary, Rats, Sex-Determining Region Y Protein - genetics, Sex-Determining Region Y Protein - metabolism, Testis-determining factor, Tumor cell lines, Tumorigenicity, Tumors