Details

Autor(en) / Beteiligte

• Pereira, Francisco B.
• Romaní, Aloia
• Ruiz, Héctor A.
• Teixeira, José A.
• Domingues, Lucília

Titel

Industrial robust yeast isolates with great potential for fermentation of lignocellulosic biomass

Ist Teil von

• Bioresource technology, 2014-06, Vol.161, p.192-199

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• •Industrial robust strains were screened for lignocellulosic ethanol production.•Screen was conducted on a real hydrolysate from Eucalyptus globulus wood.•The tolerance of the lignocellulose hydrolysate was highly variable among strains.•A correlation between final ethanol titer and furfural detoxification was found.•Distilleries showed to be a remarkable yeast source for lignocellulose fermentation. The search of robust microorganisms is essential to design sustainable processes of second generation bioethanol. Yeast strains isolated from industrial environments are generally recognised to present an increased stress tolerance but no specific information is available on their tolerance towards inhibitors that come from the pretreatment of lignocellulosic materials. In this work, a strategy for the selection of different yeasts using hydrothermal hydrolysate from Eucalyptus globulus wood, containing different concentrations of inhibitors, was developed. Ten Saccharomyces cerevisiae and four Kluyveromyces marxianus strains isolated from industrial environments and four laboratory background strains were evaluated. Interestingly, a correlation between final ethanol titer and percentage of furfural detoxification was observed. The results presented here highlight industrial distillery environments as a remarkable source of efficient yeast strains for lignocellulosic fermentation processes. Selected strains were able to resourcefully degrade furfural and HMF inhibitors, producing 0.8g ethanol/Lh corresponding to 94% of the theoretical yield.