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•18% KOH had the potential extraction effect of bamboo hemicelluloses.•Some insoluble hemicellulose presented in the hydrolysate.•Low DP hemicelluloses appeared in the pretreated substrate.•Soluble species in pretreated substrate increased with pretreatment time.
To elucidate the hemicelluloses degradation and dissolution during hydrothermal pretreatment, hemicelluloses separated from both hydrolysate and pretreated substrate were investigated. Along with the pretreatment proceeding, some hemicelluloses fractions dissolved and diffused into the bulk liquor; MW (molecular weight) of these hemicelluloses fractions increased first and then decreased as well as amount of the fractions. Based on the definition of MW of the soluble hemicellulose, it has been concluded that some insoluble hemicellulose fractions appeared in the hydrolysate. In contrast, the hemicellulose degradation occurred continually and had been observed by the gradual decrease of MW of the hemicellulose isolated from pretreated substrate. Lingering dissolution at the later stage might attribute to the facts that some soluble fractions were still entrapped in the substrate. 5–15% hemicellulose fractions remained in the pretreated substrate at the later stage were composed of soluble species.