European journal of biochemistry, 1989-01, Vol.178 (3), p.779-787
1989
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Details
- Autor(en) / Beteiligte
- Titel
- Conserved epitopes on plant H1 histones recognized by monoclonal antibodies
- Ist Teil von
- European journal of biochemistry, 1989-01, Vol.178 (3), p.779-787
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1989
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A series of monoclonal antibodies specific for distinct regions of H1 histone from the plant Nicotiana tabacum were obtained from fusion experiments with spleen cells of mice immunized with tobacco nuclear extracts. These monoclonal antibodies were characterized and the evolutionary conservation of the epitopes in higher plants and animals studied by immunoblotting and enzyme‐linked immunosorbent assay (ELISA). Whereas some epitopes appear restricted to the Solanaceae plant family, others are common to all higher eukaryotes tested and even detectable on nuclear proteins of yeast. ELISA experiments performed with isolated tobacco chromatin give some indications of the differential accessibility of the epitopes after interaction of H1 histone with the nucleosome.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-2956eISSN: 1432-1033DOI: 10.1111/j.1432-1033.1989.tb14509.xTitel-ID: cdi_proquest_miscellaneous_15337283
- Format
- –
- Schlagworte
- Analytical, structural and metabolic biochemistry, Antibodies, Monoclonal, Antigen-Antibody Reactions, Biological and medical sciences, Biological Evolution, Cell Nucleus - analysis, Chromatin - analysis, Enzyme-Linked Immunosorbent Assay, Epitopes - analysis, Fundamental and applied biological sciences. Psychology, Histones - genetics, Histones - immunology, Holoproteins, Molecular Weight, Nicotiana - genetics, Nicotiana tabacum, Nuclear proteins, Plants - genetics, Plants, Toxic, Proteins, Species Specificity