Details

Autor(en) / Beteiligte

• Lanari, Claudia
• Kordon, Edith
• Molinolo, Alfredo
• Pasqualini, Christiane Dosne
• Charreau, Eduardo H.

Titel

Mammary adenocarcinomas induced by medroxyprogesterone acetate: Hormone dependence and egf receptors of BALB/c In vivo sublines

Ist Teil von

• International journal of cancer, 1989-05, Vol.43 (5), p.845-850

Ort / Verlag

New York: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

1989

Link zum Volltext

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• Mammary adenocarcinomas were induced by medroxyprogesterone acetate (MPA) in female BALB/c mice. From 5 primary tumors, 9 different sublines were established by s.c. transplantation into syngeneic female mice; these developed after a long latent period (4‐12 months). Each subline was transplanted both into 4 mice treated with 40mg of MPA depot (s.c. contralaterally to the tumor inoculum) and into 4 non‐treated mice. Of the 9 sublines, 6 proved to be hormonedependent (MPA‐D) and 3 hormone‐independent or autonomous (MPA‐I). However, even the autonomous lines, when treated with MPA, showed a slight increase in growth. All MPA‐D lines had a high content of ER (20‐254 fmoles/mg of protein), PR (63‐710), PRL‐R (44‐74) and low or nondetectable EGF‐R. Of the 3 MPA‐I sublines that were studied, 2 showed a high content of ER (16‐125), PR (27‐708), PRL‐R (19‐70) and EGF‐R (29‐65) while the other one had a low content of ER (0‐36), PR (0‐13), no EGF‐R and moderate PRL‐R (15‐52). Spontaneous mammary tumors of BALB/c and C3H origin, which also showed an MPA‐l pattern of tumor growth, had high levels of EGF‐R. We postulate that MPA has a direct effect on mammary tumor cells in MPA‐D lines and that the expression of EGF‐R is correlated with an autonomous pattern of growth.