Details

Autor(en) / Beteiligte

• Matuszewski, Michal
• Sochacka, Elzbieta

Titel

Stability studies on the newly discovered cyclic form of tRNA N(6)-threonylcarbamoyladenosine (ct(6)A)

Ist Teil von

• Bioorganic & medicinal chemistry letters, 2014-06, Vol.24 (12), p.2703-2706

Ort / Verlag

England

Erscheinungsjahr

2014

Quelle

Elsevier ScienceDirect

Beschreibungen/Notizen

• A cyclic form of N(6)-threonylcarbamoyladenosine bearing an oxazolone moiety (ct(6)A) was discovered very recently at the position 37 in several tRNA sequences. Our study on the synthesized 5',3',2'-O-acetylated derivative of ct(6)A confirmed high stability of the modified nucleoside under physiological conditions (PBS buffer, pH 7.4) and revealed remarkable stability of the oxazolone ring in acidic (100mM HCl, pH 1) and basic (0.1mM NaOH, pH 10) conditions. This feature may allow for the post-synthetic conversion of t(6)A into ct(6)A in assembled oligoribonucleotides.