Details

Autor(en) / Beteiligte

• GUIHOT, Amélie
• LUYT, Charles-Edouard
• AGUT, Henri
• MUSSET, Lucile
• ZOOROB, Rima
• KIRILOVKSY, Amos
• COMBADIERE, Béhazine
• VAN DER WERF, Sylvie
• AUTRAN, Brigitte
• CARCELAIN, Guislaine
• PARROT, Antoine
• ROUSSET, Dominique
• CAVAILLON, Jean-Marc
• BOUTOLLEAU, David
• FITTING, Catherine
• PAJANIRASSA, Priya
• MALLET, Alain
• FARTOUKH, Muriel

Titel

Low Titers of Serum Antibodies Inhibiting Hemagglutination Predict Fatal Fulminant Influenza A(H1N1) 2009 Infection

Ist Teil von

• American journal of respiratory and critical care medicine, 2014-05, Vol.189 (10), p.1240-1249

Ort / Verlag

New York, NY: American Thoracic Society

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The biology of fatal pandemic influenza infection remains undefined. To characterize the virologic and immune parameters associated with severity or death in patients who required mechanical ventilation for A(H1N1) 2009 pneumonia of various degrees of severity during the two waves of the 2009-2011 pandemic in Paris, France. This multicenter study included 34 unvaccinated patients with very severe or fatal confirmed influenza A(H1N1) infections. It analyzed plasma A(H1N1) 2009 reverse-transcriptase polymerase chain reaction, hemagglutinin 222G viral mutation, and humoral and cellular immune responses to the virus, assessed in hemagglutination inhibition (HI), microneutralization, ELISA, lymphoproliferative, ELISpot IFN-γ, and cytokine and chemokine assays. The patients' median age was 35 years. Influenza A(H1N1) 2009 viremia was detected in 4 of 34 cases, and a 222G hemagglutinin mutation in 7 of 17 cases, all of them with sequential organ failure assessment greater than or equal to 8. HI antibodies were detectable in 19 of 26 survivors and undetectable in all six fatal fulminant cases. ELISA and microneutralization titers were concordant. B-cell immunophenotyping and plasma levels of immunoglobulin classes did not differ between patients who survived and died. After immune complex dissociation, influenza ELISA serology became strongly positive in the bronchoalveolar lavage of the two fatal cases tested. H1N1-specific T-cell responses in lymphoproliferative and IFN-γ assays were detectable in survivors' peripheral blood, and lymphoproliferative assays were negative in the three fatal cases tested. Plasma levels of IL-6 and IL-10 were high in fatal cases and correlated with severity. Finally, a negative HI serology 4 days after the onset of influenza symptoms predicted death from fulminant influenza (P = 0.04). Early negative A(H1N1) 2009 HI serology can predict death from influenza. This negative serology in fatal cases in young adults reflects the trapping of anti-H1N1 antibodies in immune complexes in the lungs, associated with poor specific helper T-cell response. Clinical trial registered with www.clinicaltrials.gov (NCT 01089400).