Details

Autor(en) / Beteiligte

• Sehgal, Lalit
• Mukhopadhyay, Amitabha
• Rajan, Anandi
• Khapare, Nileema
• Sawant, Mugdha
• Vishal, Sonali S
• Bhatt, Khyati
• Ambatipudi, Srikant
• Antao, Noelle
• Alam, Hunain
• Gurjar, Mansa
• Basu, Srikanta
• Mathur, Rohit
• Borde, Lalit
• Hosing, Amol S
• Vaidya, Milind M
• Thorat, Rahul
• Samaniego, Felipe
• Kolthur-Seetharam, Ullas
• Dalal, Sorab N

Titel

14-3-3γ-Mediated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo

Ist Teil von

• Journal of cell science, 2014-05, Vol.127 (Pt 10), p.2174-2188

Ort / Verlag

England

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The regulation of cell-cell adhesion is important for the processes of tissue formation and morphogenesis. Here, we report that loss of 14-3-3γ leads to a decrease in cell-cell adhesion and a defect in the transport of plakoglobin and other desmosomal proteins to the cell border in HCT116 cells and cells of the mouse testis. 14-3-3γ binds to plakoglobin in a PKCμ-dependent fashion, resulting in microtubule-dependent transport of plakoglobin to cell borders. Transport of plakoglobin to the border is dependent on the KIF5B-KLC1 complex. Knockdown of KIF5B in HCT116 cells, or in the mouse testis, results in a phenotype similar to that observed upon 14-3-3γ knockdown. Our results suggest that loss of 14-3-3γ leads to decreased desmosome formation and a decrease in cell-cell adhesion in vitro, and in the mouse testis in vivo, leading to defects in testis organization and spermatogenesis.