Details

Autor(en) / Beteiligte

• Gschwendt, M
• Fuerstenberger, G
• Kittstein, W
• Besemfelder, E
• Hull, W E
• Hagedorn, H
• Opferkuch, HJ
• Marks, F

Titel

Generation of the arachidonic acid metabolite 8-HETE by extracts of mouse skin treated with phorbol ester in vivo; identification by H-N.M.R. and GC-MS spectroscopy

Ist Teil von

• Carcinogenesis (New York), 1986-01, Vol.7 (3), p.449-455

Erscheinungsjahr

1986

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• After a single in vivo application of 20 nmol of the "complete" tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) or of the "incomplete" promoter 12-O-retinoylphorbol-13-acetate (RPA) to the back skin of mice, a major arachidonic acid metabolite (AAMX) was found in cell-free epidermal preparations after addition of arachidonic acid (AA). Studies with cyclooxygenase and lipoxygenase inhibitors indicated that this metabolite is a lipoxygenase product. The metabolite has been purified by sequential use of t.l.c. and h.p.l.c. and identified as 8-hydroxy-5Z,9E,11Z,14Z-eicosatetraenoic acid (8-HETE) by means of GC-MS and super(1)H-n.m.r. spectroscopy.