Details

Autor(en) / Beteiligte

• Cheng, Cheanyeh
• Chang, Kuo-Chung

Titel

Development of immobilized cellulase through functionalized gold nano-particles for glucose production by continuous hydrolysis of waste bamboo chopsticks

Ist Teil von

• Enzyme and microbial technology, 2013-12, Vol.53 (6-7), p.444-451

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• •Cellulase was immobilized on silica through functionalized gold nano-particle.•Continuous hydrolysis of waste bamboo chopsticks powder gave 82.9% conversion rate to glucose.•Immobilized cellulase can be reused at least 6 cycles for a period of 90 days with no loss of activity. Cellulase immobilized on silica through the assistance of l-cysteine functionalized gold nano-particle was applied for the continuous hydrolysis of waste bamboo chopsticks powder to produce glucose. The optimal conditions for the continuous hydrolysis were pH 8.0, 50°C. A 4-day reaction with an initial 0.3gL−1 waste bamboo chopsticks powder, a feed containing 0.2gL−1 waste bamboo chopsticks powder at a continuous feed and draw rate of 0.5mLmin−1, and an enzyme loading of 40mgcellulase(gsilica)−1, has 72.0–76.6% conversion rates of repeated hydrolyses that correspond to a total production of 630.5–671.2mg glucose and are much better than batch hydrolyses. At higher enzyme loading (117mgcellulase(gsilica)−1), higher initial concentration (0.5gL−1), and higher feed concentration (0.42gL−1) the conversion rate increases to 82.9% and a total amount of 1418mgglucose. The immobilized cellulase can be recovered easily by filtration and used repeatedly at least 6 times over a period more than 90 days with a recovered activity approximately the same as or better than previous reactions. Thus the process is promising for scaling up.