Details

Autor(en) / Beteiligte

• Carvalho, Daniela Cajado
• Duzzi, Bruno
• Kuniyoshi, Alexandre Kazuo
• Fioramonte, Mariana
• Gozzo, Fábio Cesar
• Melo, Robson L.
• Tambourgi, Denise Vilarinho
• Rioli, Vanessa
• Portaro, Fernanda Calheta

Titel

Insights into scorpion venom peptides: Alternative processing of β-KTx propeptide from Tityus serrulatus venom results in a new naturally occurring thimet oligopeptidase inhibitor

Ist Teil von

• Peptides (New York, N.Y. : 1980), 2013-02, Vol.40, p.30-33

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• ► New pentapeptide KEILG from Tityus serrulatus venom is a EP24.15 inhibitor. ► A selective tool to differentiate EP 24.15 from EP24.16. ► Substitution of one residue affects the mechanism of inhibition. ► The sequence is homologue to the propeptide portion of βKTx. ► The presence of KEILG suggests a new processing of propeptides. Most functions attributed to Tityus serrulatus venom (TsV) are related to active molecules on ion-channels; however, here we describe a new pentapeptide that was discovered through enzymatic assay selection using EP24.15. The primary structure analysis revealed the sequence KEXXG (X means Ile or Leu), similar to the sequence present in the β-KTX propeptide described from the venom of Tityus spp. We confirmed through HPLC analysis that KEILG is the peptide present in TsV, but that KELLG also inhibits EP24.15 although through different mechanisms.