Details

Autor(en) / Beteiligte

• Edwards, Chris
• Bhaduri, Basanta
• Nguyen, Tan
• Griffin, Benjamin G
• Pham, Hoa
• Kim, Taewoo
• Popescu, Gabriel
• Goddard, Lynford L

Titel

Effects of spatial coherence in diffraction phase microscopy

Ist Teil von

• Optics express, 2014-03, Vol.22 (5), p.5133-5146

Ort / Verlag

United States

Erscheinungsjahr

2014

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Quantitative phase imaging systems using white light illumination can exhibit lower noise figures than laser-based systems. However, they can also suffer from object-dependent artifacts, such as halos, which prevent accurate reconstruction of the surface topography. In this work, we show that white light diffraction phase microscopy using a standard halogen lamp can produce accurate height maps of even the most challenging structures provided that there is proper spatial filtering at: 1) the condenser to ensure adequate spatial coherence and 2) the output Fourier plane to produce a uniform reference beam. We explain that these object-dependent artifacts are a high-pass filtering phenomenon, establish design guidelines to reduce the artifacts, and then apply these guidelines to eliminate the halo effect. Since a spatially incoherent source requires significant spatial filtering, the irradiance is lower and proportionally longer exposure times are needed. To circumvent this tradeoff, we demonstrate that a supercontinuum laser, due to its high radiance, can provide accurate measurements with reduced exposure times, allowing for fast dynamic measurements.