Details

Autor(en) / Beteiligte

• Kusama, Yuta
• Tanushi, Yuichiro
• Yokoyama, Masami
• Kawakami, Ryosuke
• Hibi, Terumasa
• Kozawa, Yuichi
• Nemoto, Tomomi
• Sato, Shunichi
• Yokoyama, Hiroyuki

Titel

7-ps optical pulse generation from a 1064-nm gain-switched laser diode and its application for two-photon microscopy

Ist Teil von

• Optics express, 2014-03, Vol.22 (5), p.5746-5753

Ort / Verlag

United States

Erscheinungsjahr

2014

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• In this study, we investigated the picosecond optical pulse generation from a 1064-nm distributed feedback laser diode under strong gain switching. The spectrum of the generated optical pulses was manipulated in two different ways: (i) by extracting the short-wavelength components of the optical pulse spectrum and (ii) by compensating for spectral chirping in the extracted mid-spectral region. Both of these methods shortened the optical pulse duration to approximately 7 ps. These optical pulses were amplified to over 20-kW peak power for two-photon microscopy. We obtained clear two-photon images of neurons in a fixed brain slice of H-line mouse expressing enhanced yellow fluorescent protein. Furthermore, a successful experiment was also confirmed for in vivo deep region H-line mouse brain neuron imaging.