Details

Autor(en) / Beteiligte

• Kim, JC
• Park, J-C
• Kim, S-H
• Im, G-I
• Kim, B-S
• Lee, J-B
• Choi, E-Y
• Song, J-S
• Cho, K-S
• Kim, C-S

Titel

Treatment of FGF-2 on stem cells from inflamed dental pulp tissue from human deciduous teeth

Ist Teil von

• Oral diseases, 2014-03, Vol.20 (2), p.191-204

Ort / Verlag

Denmark: Blackwell Publishing Ltd

Erscheinungsjahr

2014

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Objective The purposes of this study were to isolate and characterize stem cells from inflamed pulp tissue of human functional deciduous teeth (iSHFD) and to evaluate the influence of fibroblastic growth factor‐2 (FGF‐2) on the regenerative potential. Materials and methods We successfully isolated mesenchymal stem cells (MSCs) from the inflamed dental pulp tissue of human deciduous teeth and demonstrated that their regenerative potential could be enhanced by the application of FGF‐2 (20 ng ml−1) during ex vivo expansion. Isolated stem cells expanded in FGF‐2 were characterized using a colony‐forming assay, proliferation, migration, in vitro differentiation, in vivo ectopic transplantation assay, and gene expression profiling. Results MSCs isolated from the inflamed pulp tissue of functional deciduous teeth potentially possess the qualities of those from human exfoliated deciduous teeth. FGF‐2 applied to iSHFD during expansion enhanced the colony‐forming efficiency of these cells, increased their proliferation and migration potential, and reduced their differentiation potential in vitro. However, the ectopic transplantation of iSHFD/FGF‐2 in vivo increased the formation of dentin‐like material. Conclusion FGF‐2 expansion of stem cells from inflamed pulp tissues of human deciduous teeth can be a good source of stem cells for future clinical applications and a novel way of using discarded inflamed tissues.