Details

Autor(en) / Beteiligte

• Thangam, Ramar
• Suresh, Veeraperumal
• Kannan, Soundarapandian

Titel

Optimized extraction of polysaccharides from Cymbopogon citratus and its biological activities

Ist Teil von

• International journal of biological macromolecules, 2014-04, Vol.65, p.415-423

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• •Extraction of hot water-soluble polysaccharides (HWSPs) from Cymbopogon citratus.•Response surface methodology was used to optimize the extraction procedure.•Ratio of dried plant powder to water, extraction time and temperature were discussed.•The best extraction methods were 99.66°C, 33.11g/mL ratio of dried plant materials to water, and 113.81min.•In vitro biological activities were evaluated for HWSPs. In this study the extraction of hot water soluble polysaccharides (HWSPs) from Cymbopogon citratus using hot water decoction was discussed. Response surface methodology (RSM) based on a three level, three variable central composite rotatable design (CCRD), was employed to obtain best possible combination of extraction time (X1: 30–180min), extraction temperature (X2: 70–100°C) and water to the raw material ratio (X3: 10–60) for maximum HWSPs extraction. The optimum extraction conditions were as follows: extraction time was around 113.81min, extraction temperature at 99.66°C and the ratio of water to raw material was 33.11g/mL. Under these conditions, the experimental yield was 13.24±0.23%, which is well in close agreement with the value predicted by RSM model yield (13.19%). The basic characterization of HWSPs was determined by using the FTIR. These preliminary in vitro biological studies indicated that lemongrass polysaccharides were useful for anticancer therapy.