Details

Autor(en) / Beteiligte

• Xu, Jiao-Jiao
• Wu, Xia
• Li, Man-Mei
• Li, Guo-Qiang
• Yang, Yi-Ting
• Luo, Hu-Jie
• Huang, Wei-Huang
• Chung, Hau Yin
• Ye, Wen-Cai
• Wang, Guo-Cai
• Li, Yao-Lan

Titel

Antiviral Activity of Polymethoxylated Flavones from “Guangchenpi”, the Edible and Medicinal Pericarps of Citrus reticulata ‘Chachi’

Ist Teil von

• Journal of agricultural and food chemistry, 2014-03, Vol.62 (10), p.2182-2189

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The present study found that the supercritical fluid extract of “Guangchenpi” possessed in vitro antiviral activity against respiratory syncytial virus (RSV). Bioassay-guided isolation and identification of this extract led to obtain five active polymethoxylated flavones (1–5). Cytopathic effect (CPE) reduction assay exhibited that tangeretin (2) and nobiletin (3), two major polymethoxylated flavones in the extract, possessed better anti-RSV effect comparable to the positive control ribavirin. Plaque reduction assay revealed that tangeretin dose-dependently inhibited RSV-induced plaque formation on the HEp-2 cells. This polymethoxylated flavone mainly affected the intracellular replication of RSV, and it also could inhibit RSV entry into the HEp-2 cells. Further investigations with quantitative real-time PCR and confocal and Western blot assays indicated that tangeretin downregulated the expression of RSV phosphoprotein (P protein). Results suggest the potential application of the supercritical fluid extract of “Guangchenpi” and tangeretin in the treatment and the prevention of RSV infection.