Details

Autor(en) / Beteiligte

• Ashraf, Shazia
• Gee, Heon Yung
• Woerner, Stephanie
• Xie, Letian X
• Vega-Warner, Virginia
• Lovric, Svjetlana
• Fang, Humphrey
• Song, Xuewen
• Cattran, Daniel C
• Avila-Casado, Carmen
• Paterson, Andrew D
• Nitschke, Patrick
• Bole-Feysot, Christine
• Cochat, Pierre
• Esteve-Rudd, Julian
• Haberberger, Birgit
• Allen, Susan J
• Zhou, Weibin
• Airik, Rannar
• Otto, Edgar A
• Barua, Moumita
• Al-Hamed, Mohamed H
• Kari, Jameela A
• Evans, Jonathan
• Bierzynska, Agnieszka
• Saleem, Moin A
• Bockenhauer, Detlef
• Kleta, Robert
• El Desoky, Sherif
• Hacihamdioglu, Duygu O
• Gok, Faysal
• Washburn, Joseph
• Wiggins, Roger C
• Choi, Murim
• Lifton, Richard P
• Levy, Shawn
• Han, Zhe
• Salviati, Leonardo
• Prokisch, Holger
• Williams, David S
• Pollak, Martin
• Clarke, Catherine F
• Pei, York
• Antignac, Corinne
• Hildebrandt, Friedhelm

Titel

ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ sub( 10) biosynthesis disruption

Ist Teil von

• The Journal of clinical investigation, 2013-12, Vol.123 (12), p.5179-5179

Erscheinungsjahr

2013

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Identification of single-gene causes of steroid-resistant nephrotic syndrome (SRNS) has furthered the understanding of the pathogenesis of this disease. Here, using a combination of homozygosity mapping and whole human exome resequencing, we identified mutations in the aarF domain containing kinase 4 (ADCK4) gene in 15 individuals with SRNS from 8 unrelated families. ADCK4 was highly similar to ADCK3, which has been shown to participate in coenzyme Q sub( 10) (CoQ sub( 10)) biosynthesis. Mutations in ADCK4 resulted in reduced CoQ10 levels and reduced mitochondrial respiratory enzyme activity in cells isolated from individuals with SRNS and transformed lymphoblasts. Knockdown of adck4 in zebrafish and Drosophila recapitulated nephrotic syndrome-associated phenotypes. Furthermore, ADCK4 was expressed in glomerular podocytes and partially localized to podocyte mitochondria and foot processes in rat kidneys and cultured human podocytes. In human podocytes, ADCK4 interacted with members of the CoQ sub( 10) biosynthesis pathway, including COQ6, which has been linked with SRNS and COQ7. Knockdown of ADCK4 in podocytes resulted in decreased migration, which was reversed by CoQ sub( 10) addition. Interestingly, a patient with SRNS with a homozygous ADCK4 frameshift mutation had partial remission following CoQ sub( 10) treatment. These data indicate that individuals with SRNS with mutations in ADCK4 or other genes that participate in CoQ sub( 10) biosynthesis may be treatable with CoQ sub( 10).