Details

Autor(en) / Beteiligte

• Morandi, Fabio
• Venturi, Consuelo
• Rizzo, Roberta
• Castellazzi, Massimiliano
• Baldi, Eleonora
• Caniatti, Maria Luisa
• Tola, Maria Rosaria
• Granieri, Enrico
• Fainardi, Enrico
• Uccelli, Antonio
• Pistoia, Vito

Titel

Intrathecal Soluble HLA-E Correlates with Disease Activity in Patients with Multiple Sclerosis and may Cooperate with Soluble HLA-G in the Resolution of Neuroinflammation

Ist Teil von

• Journal of neuroimmune pharmacology, 2013-09, Vol.8 (4), p.944-955

Ort / Verlag

Boston: Springer US

Erscheinungsjahr

2013

Link zum Volltext

Quelle

SpringerLink

Beschreibungen/Notizen

• Expression and function of the immunoregulatory molecule HLA-E was investigated in patients with relapsing-remitting (RR) multiple sclerosis (MS). Serum and cerebrospinal fluid (CSF) soluble (s)HLA-E and -G levels were measured by ELISA in 80 RRMS patients. Controls were patients with other inflammatory neurological disorders (OIND, n  = 81) and noninflammatory neurological disorders (NIND, n  = 86). Serum sHLA-E concentrations were higher in RRMS than in NIND patients only. CSF sHLA-E concentrations were higher in RRMS than controls. Increased CSF sHLA-E levels were detected in MRI inactive and clinically stable RRMS patients. sHLA-E intrathecal synthesis (ITS) was higher in RRMS than controls, and the number of patients with sHLA-E ITS above cut-off was higher i) in MS than controls, and ii) in clinically stable than clinically active MS patients. sHLA-E CSF levels and ITS correlated with i) the same sHLA-G parameters, and ii) disease duration. HLA-E expression and co-expression with CD markers were investigated in MS plaques from three different cases by immunohistochemistry and confocal microscopy, respectively. Infiltrating T lymphocytes and macrophages, as well as resident microglial cells and astrocytes expressed HLA-E. CSF samples from MS patients were finally tested for inhibitory activity of in vitro CTL and NK cell mediated cytotoxicity. sHLA-E + were more effective than sHLA-E − CSF samples in such inhibition. Maximum inhibition was achieved with sHLA-E + /sHLA–G + CSF samples In conclusion , increased sHLA-E CSF levels may play an immunomodulatory role in MS, contributing to the inhibition of intrathecal inflammatory response. The potential of sHLA-E as biomarker of MS activity warrants further investigation.