Details

Autor(en) / Beteiligte

• Fernández-Trujillo, M.A.
• García-Rosado, E.
• Alonso, M.C.
• Castro, D.
• Álvarez, M.C.
• Béjar, J.

Titel

Mx1, Mx2 and Mx3 proteins from the gilthead seabream (Sparus aurata) show in vitro antiviral activity against RNA and DNA viruses

Ist Teil von

• Molecular immunology, 2013-12, Vol.56 (4), p.630-636

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2013

Quelle

ScienceDirect Pay Per View(PPV) Titles

Beschreibungen/Notizen

• •The antiviral activity of Mx proteins from gilthead seabream has been evaluated.•Mx1 has activity against VHSV and LCDV; Mx2 against LCDV and ESV; Mx3 against VHSV.•These differences support the fine-tuning of virus–host interaction. Mx proteins are important components of the antiviral innate immune response mediated by type I interferon. Classically, these proteins have been considered to be triggered by viral RNA, thus showing activity against RNA viruses. Actually, three Mx proteins (SauMx1, SauMx2 and SauMx3) from gilthead seabream (Sparus aurata) have previously shown antiviral activity against a dsRNA virus: the infectious pancreatic necrosis virus (IPNV) in vitro. For further characterizing their antiviral spectrum, the activity of SauMx proteins were tested against three different viral pathogens of fish: the lymphocystis disease virus (LCDV, a dsDNA virus), a pathogen of gilthead seabream; the viral haemorrhagic septicaemia virus (VHSV, a ssRNA virus), to which gilthead seabream is considered a reservoir species; and the European sheatfish virus (ESV, a dsDNA virus), that has not been detected in gilthead seabream to date. Three clonal populations of CHSE-214 cells developed in a previous study, stably expressing SauMx1, SauMx2 and SauMx3, respectively, were challenged with the three viruses. Results combining cytopathic effects and virus yield reduction assays showed that SauMx1 protected the cells against VHSV and LCDV, SauMx2 protected against ESV and LCDV, and SauMx3 showed activity only against VHSV. This study, besides confirming the antiviral activity of the three gilthead seabream Mx proteins, is the first report of the protective effect of a fish Mx against DNA viruses. Additionally, it discloses a clear specificity between Mx proteins and virus targets, supporting the idea that the relationship between virus and Mx proteins is finely tuned.