Metabolism, clinical and experimental, 2014-03, Vol.63 (3), p.312-317
2014
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- Autor(en) / Beteiligte
- Titel
- Fibroblast growth factor-21 is expressed in neonatal and pheochromocytoma-induced adult human brown adipose tissue
- Ist Teil von
- Metabolism, clinical and experimental, 2014-03, Vol.63 (3), p.312-317
- Ort / Verlag
- New York, NY: Elsevier Inc
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Objective In rodents, brown (BAT) and white (WAT) adipose tissues are targets and expression sites for fibroblast growth factor-21 (FGF21). In contrast, human WAT expresses negligible levels of FGF21. We examined FGF21 expression in human BAT samples, including the induced BAT found in adult patients with pheochromocytoma, and interscapular and visceral BAT from newborns. Methods The expression of FGF21 and uncoupling protein-1 (UCP1, a brown adipocyte marker), was determined by quantitative real-time-PCR and immunoblotting. The transcript levels of marker genes for developmentally-programmed BAT (zinc-finger-protein of the cerebellum-1, ZIC1) and inducible-BAT (cluster of differentiation-137, CD137) were also determined. Results FGF21 and UCP1 are significantly expressed in visceral adipose tissue from pheochromocytoma patients, but not in visceral fat from healthy individuals. In neonates, FGF21 and UCP1 are both expressed in visceral and interscapular fat, and their expression levels show a significant positive correlation. Marker gene expression profiles suggest that inducible BAT is present in visceral fat from pheochromocytoma patients and neonates, whereas developmentally-programmed BAT is present in neonatal interscapular fat. Conclusions Human BAT, but not WAT, expresses FGF21. The expression of FGF21 is especially high in inducible, also called beige/brite, neonatal BAT, but it is also found in the interscapular, developmentally-programmed, BAT of neonates.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0026-0495eISSN: 1532-8600DOI: 10.1016/j.metabol.2013.11.014Titel-ID: cdi_proquest_miscellaneous_1500698586
- Format
- –
- Schlagworte
- 4-1BB Ligand - genetics, 4-1BB Ligand - metabolism, Adipocytes, Brown - metabolism, Adipose Tissue, Brown - metabolism, Adipose Tissue, White - metabolism, Adrenal Gland Neoplasms - genetics, Adrenal Gland Neoplasms - metabolism, Adrenals. Adrenal axis. Renin-angiotensin system (diseases), Adult, Aged, Beige adipocyte, Biological and medical sciences, Brite adipocyte, Diabetes, Endocrinology & Metabolism, Endocrinopathies, Feeding. Feeding behavior, Female, Fibroblast Growth Factors - genetics, Fibroblast Growth Factors - metabolism, Fundamental and applied biological sciences. Psychology, Humans, Infant, Newborn, Ion Channels - genetics, Ion Channels - metabolism, Male, Medical sciences, Middle Aged, Mitochondrial Proteins - genetics, Mitochondrial Proteins - metabolism, Non tumoral diseases. Target tissue resistance. Benign neoplasms, Obesity, Pheochromocytoma - genetics, Pheochromocytoma - metabolism, Transcription Factors - genetics, Transcription Factors - metabolism, Transcriptome - genetics, Uncoupling Protein 1, Vertebrates: anatomy and physiology, studies on body, several organs or systems