Applied microbiology and biotechnology, 2014-01, Vol.98 (2), p.777-783
2014
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Overexpression of a Metarhizium robertsii HSP25 gene increases thermotolerance and survival in soil
- Ist Teil von
- Applied microbiology and biotechnology, 2014-01, Vol.98 (2), p.777-783
- Ort / Verlag
- Berlin/Heidelberg: Springer-Verlag
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Temperature extremes are an important adverse factor limiting the effectiveness of microbial pest control agents. They reduce virulence and persistence in the plant root-colonizing insect pathogen Metarhizium robertsii. Small heat shock proteins have been shown to confer thermotolerance in many organisms. In this study, we report on the cloning and characterization of a small heat shock protein gene hsp25 from M. robertsii. hsp25 expression was upregulated when the fungus was grown at extreme temperatures (4, 35, and 42 °C) or in the presence of oxidative or osmotic agents. Expression of hsp25 in Escherichia coli increased bacterial thermotolerance confirming that hsp25 encodes a functional heat shock protein. Overexpressing hsp25 in M. robertsii increased fungal growth under heat stress either in nutrient-rich medium or on locust wings and enhanced the tolerance of heat shock-treated conidia to osmotic stress. In addition, overexpression of hsp25 increased the persistence of M. robertsii in rhizospheric soils in outdoor microcosms, though it did not affect survival in bulk soil, indicating that M. robertsii's survival in soil is dependent on interactions with plant roots.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0175-7598eISSN: 1432-0614DOI: 10.1007/s00253-013-5360-5Titel-ID: cdi_proquest_miscellaneous_1496886983
- Format
- –
- Schlagworte
- Abiotic stress, Agricultural biotechnology, Antimicrobial agents, Applied Genetics and Molecular Biotechnology, Ascomycota, Biomedical and Life Sciences, Biotechnology, Cloning, conidia, E coli, Escherichia coli, Fungi, Gene Expression, Gene Expression Profiling, gene expression regulation, gene overexpression, Genes, Genetic aspects, Heat, Heat shock proteins, heat stress, Heat tolerance, Heat-Shock Proteins - genetics, Heat-Shock Proteins - metabolism, Insects, Life Sciences, Metarhizium, Metarhizium - genetics, Metarhizium - physiology, Metarhizium - radiation effects, Microbial genetics, Microbial Genetics and Genomics, microbial growth, Microbial Viability - drug effects, Microbiological research, Microbiology, osmotic stress, Pathogens, Pest control, Physiological aspects, Protein expression, roots, Seeds, soil, Soil Microbiology, Soil temperature, Studies, Temperature, Temperature effects, Virulence, wings