Details

Autor(en) / Beteiligte

• Je, Eun Mi
• Yoo, Nam Jin
• Kim, Yoo Jin
• Kim, Myung Shin
• Lee, Sug Hyung

Titel

Mutational analysis of splicing machinery genes SF3B1, U2AF1 and SRSF2 in myelodysplasia and other common tumors

Ist Teil von

• International journal of cancer, 2013-07, Vol.133 (1), p.260-265

Ort / Verlag

Hoboken, NJ: Wiley-Blackwell

Erscheinungsjahr

2013

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Recurrent somatic mutations in splicing machinery components, including SF3B1, U2AF1 and SRSF2 genes have recently been reported in myelodysplastic syndromes (MDS). Such a recurrent nature strongly suggests that these mutations play important roles in tumor development. To see whether SF3B1, U2AF1 and SRSF2 mutations occur in other human tumors besides MDS, we analyzed the hotspot mutation regions of these genes in 2,345 tumor tissues from various origins (61 MDS, other 616 hematologic tumors, 1,421 epithelial tumors and 247 non‐epithelial stromal tumors) by single‐strand conformation polymorphism analysis. We found SF3B1, U2AF1 and SRSF2 mutations in 5 (8.2%), 12 (19.7%) and 8 (13.1%) of 61 MDS, respectively. We also confirmed these mutations in other myeloid neoplasia, including de novo acute myelogenous leukemia (AML), chronic myelomonocytic leukemia and MDS/myeloproliferative disorder. In addition, we discovered that the SRSF2 gene was mutated in two childhood acute lymphoblastic leukemias (childhood ALL) (1.5%). In solid tumors, we found SF3B1 mutations in gastric and prostate cancers, and U2AF1 mutation in a borderline mucinous tumor of ovary, but the overall incidences of the hotspot mutation regions were very low (0.2%). Our data suggest that SF3B1, U2AF1 and SRSF2 mutations occur not only in myeloid lineage tumors but also in lymphoid lineage tumors. The data suggest that the splicing gene mutations play important roles in the pathogenesis of hematologic tumors, but rarely in solid tumors. What's new? Recurrent somatic mutations in RNA splicing machinery components have recently been reported in myelodysplastic syndromes (MDS). Here the authors analyzed tumor tissues from various origins to see whether the mutations also occur in other human tumors. The results confirmed that somatic mutations in splicing machinery components are common in MDS and acute myelogenous leukemias. The mutations also occurred in lymphoid lineage tumors such as childhood ALL, but were rare in other human tumors. The data suggested that the mutations play important roles in the pathogenesis of hematologic tumors but rarely in solid tumors.