Biomaterials, 2014-03, Vol.35 (9), p.2580-2587
2014
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Simultaneous interaction of bacteria and tissue cells with photocatalytically activated, anodized titanium surfaces
- Ist Teil von
- Biomaterials, 2014-03, Vol.35 (9), p.2580-2587
- Ort / Verlag
- Netherlands
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Photocatalytic-activation of anodized TiO2 -surfaces has been demonstrated to yield antibacterial and tissue integrating effects, but effects on simultaneous growth of tissue cells and bacteria in co-culture have never been studied. Moreover, it is unknown how human-bone-marrow-mesenchymal-stem (hBMMS) cells, laying the groundwork for integration of titanium implants in bone, respond to photocatalytic activation of anodized TiO2 -surfaces. Photocatalytically-activated, anodized titanium and titanium-alloy surfaces achieved 99.99% killing of adhering Staphylococcus epidermidis and Staphylococcus aureus , an effect that lasted for 30 days of storage in air. Surface coverage by osteoblasts was not affected by photocatalytic activation of anodized TiO2 -surfaces. Co-cultures of osteoblasts with contaminating S. epidermidis however, enhanced surface coverage on photocatalytically-activated, anodized titanium-alloy surfaces. hBMMS cells grew less on photocatalytically-activated, anodized titanium surfaces, while not at all on photocatalytically-activated, anodized titanium-alloy surfaces and did not survive the presence of contaminating staphylococci. This reduced surface coverage by hBMMS cells disappeared when photocatalytically-activated, anodized titanium-alloy surfaces were exposed to buffer for 60 min, both in absence or presence of contaminating S. aureus . Consequently, it is concluded that photocatalytically-activated, anodized titanium and titanium-alloy surfaces will effectively kill peri-operatively introduced staphylococci contaminating an implant surface and constitute an effective means for antibiotic prophylaxis in cementless fixation of orthopaedic hardware.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0142-9612eISSN: 1878-5905DOI: 10.1016/j.biomaterials.2013.12.036Titel-ID: cdi_proquest_miscellaneous_1494305419
- Format
- –
- Schlagworte
- Advanced Basic Science, Alloys - pharmacology, Anti-Bacterial Agents - pharmacology, Bone Marrow Cells - cytology, Bone Marrow Cells - drug effects, Bone Marrow Cells - radiation effects, Catalysis - drug effects, Catalysis - radiation effects, Cell Adhesion - drug effects, Cell Adhesion - radiation effects, Cell Communication - drug effects, Cell Communication - radiation effects, Cell Line, Tumor, Cell Movement - drug effects, Cell Movement - radiation effects, Dentistry, Electrodes, Humans, Light, Mesenchymal Stromal Cells - cytology, Mesenchymal Stromal Cells - drug effects, Mesenchymal Stromal Cells - radiation effects, Microbial Sensitivity Tests, Microbial Viability - drug effects, Microbial Viability - radiation effects, Microscopy, Electron, Scanning, Staphylococcus aureus - cytology, Staphylococcus aureus - drug effects, Staphylococcus aureus - radiation effects, Staphylococcus epidermidis - cytology, Staphylococcus epidermidis - drug effects, Staphylococcus epidermidis - radiation effects, Surface Properties, Titanium - pharmacology, Water - chemistry