Details

Autor(en) / Beteiligte

• Chen, Hu
• Wang, Siqi
• Chen, Luxi
• Chen, Yaosheng
• Wu, Ming
• Zhang, Yun
• Yu, Kaifan
• Huang, Zheng
• Qin, Lijun
• Mo, Delin

Titel

MicroRNA-344 inhibits 3T3-L1 cell differentiation via targeting GSK3β of Wnt/β-catenin signaling pathway

Ist Teil von

• FEBS letters, 2014-01, Vol.588 (3), p.429-435

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2014

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• •Overexpression of miR-344 inhibited 3T3-L1 preadipocyte differentiation.•GSK3β was confirmed to be the target of miR-344.•Knockdown of endogenous GSK3β repressed 3T3-L1 preadipocyte differentiation.•Overexpression of GSK3β could overcome the effect of miR-344.•miR-344 activated Wnt/β-catenin signaling and repressed preadipocyte differentiation. Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of complex events in which microRNAs might play an essential role. In this study, we found that the overexpression of microRNA-344 (miR-344) inhibits 3T3-L1 cell differentiation and decreases triglyceride accumulation after MDI stimulation. We demonstrated that miR-344 directly targets the 3′ UTR of GSK3β (Glycogen synthase kinase 3 beta). Knockdown of GSK3β with siRNA results in inhibiting 3T3-L1 differentiation, while its overexpression restores the effect of miR-344. In addition, miR-344 elevates the level of active β-catenin, which is the downstream effector of GSK3β in the Wnt/β-catenin signaling pathway. These data indicate that miR-344 inhibits adipocyte differentiation via targeting GSK3β and subsequently activating the Wnt/β-catenin signaling pathway.