Details

Autor(en) / Beteiligte

• Chang, Cheng-Chang
• Huang, Rui-Lan
• Wang, Hui-Chen
• Liao, Yu-Ping
• Yu, Mu-Hsien
• Lai, Hung-Cheng

Titel

High Methylation Rate of LMX1A, NKX6-1, PAX1, PTPRR, SOX1, and ZNF582 Genes in Cervical Adenocarcinoma

Ist Teil von

• International journal of gynecological cancer, 2014-02, Vol.24 (2), p.201-209

Ort / Verlag

England: by the International Gynecologic Cancer Society and the European Society of Gynaecological Oncology

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• OBJECTIVEThis study aimed to investigate the status of DNA methylation of 6 genes, LMX1A, NKX6-1, PAX1, PTPRR, SOX1, and ZNF582, previously found from squamous cell carcinomas in adenocarcinomas (ACs) of the uterine cervix. METHODSWe assessed the methylation status of these genes in 40 ACs, cervical scrapings from 23 ACs, and 67 normal control cervices by real-time quantitative methylation-specific polymerase chain reaction. The results were validated by bisulfite pyrosequencing. RESULTSThe methylation levels of all the 6 genes in the ACs were significantly higher than those in normal cervical tissues, especially for PAX1, PTPRR, SOX1, and ZNF582. The odds ratios and 95% confidence intervals (CIs) of high methylation levels in PAX1, PTPRR, SOX1, and ZNF582 for the risk of developing an AC were 15.7 (95% CI, 7.0–40.6), 16.9 (95% CI, 7.6–43.0), 32.1 (95% CI, 12.1–124.3), and 25.4 (95% CI, 10.4–78.3), respectively (all P < 0.001). The methylation indices of PAX1, PTPRR, SOX1, and ZNF582 recovered from scrapings of ACs were significantly higher than in normal controls. The odds ratios of these indices for the risk of developing an AC in PAX1, PTPRR, SOX1, and ZNF582 were 6.2 (95% CI, 2.6–15.4), 12.1(95% CI, 3.8–46.4), 6.2 (95% CI, 2.6–15.8), and 20.6 (95% CI, 6.9–77.5), respectively (all P < 0.001). CONCLUSIONSCervical ACs carry aberrantly high methylation rates of PAX1, PTPRR, SOX1, and ZNF582—commonly methylated in squamous cell carcinomas—which might help for AC screening.