Details

Autor(en) / Beteiligte

• Webb, T R
• Jhurani, P
• Ng, P G

Titel

Template-primer analog as substrates for DNA polymerase

Ist Teil von

• Nucleic acids research, 1987-01, Vol.15 (10), p.3997-4006

Erscheinungsjahr

1987

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• In order to gain more understanding about the mode of action of DNA polymerase, eight related partially self-complementary "hairpin" shaped oligodeoxynucleotides were prepared. Four of the oligomers contained either 1- beta -D-arabinofuranosyluracil (ara-U) or 1- beta -D-2'deoxylofuranosyl-thymine (dxT) nucleoside analogs at their 3' termini. The authors investigated the ability of the oligomers to prime DNA synthesis in relationship to the stability of the hybridized region, the nature of the sugar terminus and the DNA polymerase used (reverse transcriptase, or polymerase alpha ). The results are discussed in relation to the mode of action of some nucleoside analog inhibitors of DNA polymerase. An understanding of the mechanism of DNA polymerase action was used to design template-primer analogs as polymerase inhibitors. Two of the oligodeoxynucleotide analogs prepared were found to be potent inhibitors of polymerase alpha .