Details

Autor(en) / Beteiligte

• Brown, E J
• Bohnsack, J F
• O'Shea, J J
• McGarr, J

Titel

Evidence of a conformational change in human fibronectin which occurs between 4 and 37 degree C

Ist Teil von

• Molecular immunology, 1987-01, Vol.24 (3), p.221-230

Erscheinungsjahr

1987

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The effects of temperatures between 4 and 37 degree C on the structure of human fibronectin (Fn) using monoclonal antibodies (MAb) with specificity for Fn have been studied. Three MAb bound to Fn in solution better at 4 than at 37 degree C, assessed by both ELISA and immunoprecipitation. It is concluded that Fn has different conformations at 4 and at 37 degree C in solution and after adherence to surfaces, as reflected by MAb binding, detergent sensitivity and protease susceptibility. This conformational difference is not associated with alterations in the physiochemical properties of the molecule and is thus a result of a less extensive intramolecular rearrangement than the conformational changes resulting from manipulations of ionic strength or pH, and from binding to collagen or hydrophobic surfaces. Nonetheless, the more subtle conformational change demonstrated here may be important for the biological properties of Fn.