Details

Autor(en) / Beteiligte

• Strader, Catherine D.
• Sigal, Irving S.
• Blake, Allan D.
• Cheung, Anne H.
• Register, R.Bruce
• Rands, Elaine
• Zemcik, Barbara A.
• Candelore, Mari Rios
• Dixon, Richard A.F.

Titel

The carboxyl terminus of the hamster β-adrenergic receptor expressed in mouse L cells is not required for receptor sequestration

Ist Teil von

• Cell, 1987-06, Vol.49 (6), p.855-863

Ort / Verlag

Cambridge, MA: Elsevier Inc

Erscheinungsjahr

1987

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The structural basis for agonist-mediated sequestration and desensitization of the β-adrenergic receptor (βAR) was examined by oligonucleotide-directed mutagenesis of the hamster βAR gene and expression of the mutant genes in mouse L cells. Treatment of these cells with the agonist isoproterenol corresponded to a desensitization of βAR activity. A mutant receptor that bound agonist but did not couple to adenylate cyclase showed a dramatically reduced sequestration response to agonist stimulation. In contrast, βAR mutants in which the C-terminus was truncated and/or in which two regions that have been proposed as phosphorylation substrates for cAMP-dependent protein kinase were removed showed normal sequestration responses. These results demonstrate that agonist-mediated sequestration of the βAR can occur in the absence of the C-terminus of the protein and reveal a strong correlation between effective coupling to G s and sequestration.