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The responses of Vitreoscilla hemoglobin-expressing hybrid aspen (Populus tremula × tremuloides) exposed to 24-h herbivory: expression of hemoglobin and stress-related genes in exposed and nonorthostichous leaves
Ist Teil von
Journal of plant research, 2013-11, Vol.126 (6), p.795-809
Ort / Verlag
Tokyo: Springer Japan
Erscheinungsjahr
2013
Quelle
MEDLINE
Beschreibungen/Notizen
The responses of transcriptome and phenolic compounds were determined with
Populus tremula
L. ×
Populus tremuloides
Michx. expressing the hemoglobin (Hb) of
Vitreoscilla
(VHb) and non-transformant (wt) line. After 24-h exposure of leaves to
Conistra vaccinii
L., the transcript levels of endogenous non-symbiotic class 1 Hb (
PttHb1
) and truncated Hb (
PttTrHb
) genes were modestly reduced and increased, respectively, in both wt and VHb-expressing line. Besides the herbivory exposed leaves showing the most significant transcriptome changes, alterations were also detected in the transcriptome of nonorthostichous leaves positioned directly above the exposed leaves. Both wt and VHb-expressing line displayed similar herbivory-induced effects on gene expression, although the extent of responses was more pronounced in the wt than in the VHb-expressing line. The contents of phenolic compounds were not altered due to herbivory and they were alike in the wt and VHb-expressing line. In addition, we determined the relative growth rates (RGRs) of
Orthosia gothica
L.,
Ectropis crepuscularia
Denis & Schiff. and
Orgyia antiqua
L. larvae, and found no variation in the RGRs between the lines. Thus, VHb-expressing
P. tremula
×
tremuloides
lines showed to be comparable with wt in regards to the food quality of leaves.