Cancer gene therapy, 2013-11, Vol.20 (11), p.622-629
2013
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- Autor(en) / Beteiligte
- Titel
- Downregulation of G3BPs inhibits the growth, migration and invasion of human lung carcinoma H1299 cells by suppressing the Src/FAK-associated signaling pathway
- Ist Teil von
- Cancer gene therapy, 2013-11, Vol.20 (11), p.622-629
- Ort / Verlag
- New York: Nature Publishing Group US
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- G3BP is a RasGAP binding protein that is overexpressed in many human cancers. We previously reported that downregulation of G3BP suppressed cell growth and induced apoptosis in HCT116 cells. Here we report that both transient and stable knockdown of G3BP suppressed the growth, migration and invasion capability of human lung carcinoma H1299 cells. Moreover, downregulation of G3BP significantly inhibited the phosphorylation of Src, FAK and ERK, and the levels of NF-κB were also markedly decreased in H1299 cells. Knockdown of G3BP also decreased the expression of matrix metalloproteinase-2 (MMP-2), MMP-9 and plasminogen activator (uPA), and in vivo data demonstrated that downregulation of G3BP markedly inhibited the growth of H1299 tumor xenografts. Together, these data revealed that knockdown of G3BP inhibited the migration and invasion of human lung carcinoma cells through the inhibition of Src, FAK, ERK and NF-κB and decreased levels of MMP-2, MMP-9 and uPA.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0929-1903eISSN: 1476-5500DOI: 10.1038/cgt.2013.62Titel-ID: cdi_proquest_miscellaneous_1468350895
- Format
- –
- Schlagworte
- 631/154/51/391/505, 631/67/1612, 631/80/86, Animals, Apoptosis, Binding proteins, Biomedical and Life Sciences, Biomedicine, Cancer, Carcinoma, Non-Small-Cell Lung - genetics, Carcinoma, Non-Small-Cell Lung - metabolism, Carcinoma, Non-Small-Cell Lung - pathology, Carcinoma, Non-Small-Cell Lung - therapy, Carrier Proteins - genetics, Carrier Proteins - metabolism, Cell Growth Processes - genetics, Cell Line, Tumor, Cell Movement - genetics, Cellular signal transduction, Development and progression, DNA Helicases, Down-Regulation, Female, Focal adhesion kinase, Focal Adhesion Kinase 1 - antagonists & inhibitors, Focal Adhesion Kinase 1 - genetics, Focal Adhesion Kinase 1 - metabolism, Gelatinase A, Gelatinase B, Gene Expression, Gene Knockdown Techniques, Gene Therapy, Genetic aspects, Humans, Identification and classification, Lung cancer, Lung carcinoma, Lung Neoplasms - genetics, Lung Neoplasms - metabolism, Lung Neoplasms - pathology, Lung Neoplasms - therapy, Matrix metalloproteinase, Metalloproteinase, Mice, NF-kappa B - metabolism, NF-κB protein, original-article, Phosphorylation, Poly-ADP-Ribose Binding Proteins, RNA Helicases, RNA Recognition Motif Proteins, RNA, Small Interfering - genetics, RNA, Small Interfering - metabolism, Signal Transduction, Src protein, src-Family Kinases - antagonists & inhibitors, src-Family Kinases - genetics, src-Family Kinases - metabolism, Transfection, Tumors, U-Plasminogen activator, Xenograft Model Antitumor Assays, Xenografts