Details

Autor(en) / Beteiligte

• Zhu, Xuejing
• Wen, Feng
• Yang, Danyi
• Liu, Jing
• Yuan, Shuguang
• Li, Jun
• Liu, Hong
• Xu, Xiangqing
• Sun, Lin
• Liu, Fuyou

Titel

Effect of high glucose peritoneal dialysis solution on PGC-1α expression and mitochondria related oxidative injury in human peritoneal mesothelial cells

Ist Teil von

• Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2013-11, Vol.38 (11), p.1085-1091

Ort / Verlag

China

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the mechanism of mitochondrial oxidative injury induced by high glucose peritoneal dialysis solution (PDS) and the protective effect of peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α) in the mitochondria of human peritoneal mesothelial cells (HPMC) in the high glucose ambience. HPMC was cultured in a PDS containing 1.5%, 2.5% and 4.25% glucose for 24 hours. Western blot analysis was used to detect PGC-1α expression. MitoSOX? Red staining, respiratory chain complexes and antioxidant enzyme activities were determined. The activities of respiratory chain complex III and antioxidant enzymes decreased significantly in a concentration- and time-dependent manner, along with the increased production of mitochondrial reactive oxygen species (ROS) and cellular apoptosis. In addition, protein expression of PGC-1α was also decreased in the high glucose PDS ambience. High glucose PDS might inhibit PGC-1α expression, resulting in the inhibition of mitochondrial function and incr