Hepatobiliary & pancreatic diseases international, 2013-12, Vol.12 (6), p.630-636
2013
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- Autor(en) / Beteiligte
- Titel
- Upregulation of caveolin-1 and SR-B1 in mice with non-alcoholic fatty liver disease
- Ist Teil von
- Hepatobiliary & pancreatic diseases international, 2013-12, Vol.12 (6), p.630-636
- Ort / Verlag
- Singapore: Elsevier B.V
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- BACKGROUND:Non-alcoholic fatty liver disease(NAFLD)is one of the most frequent causes of liver diseases,with markedly increased prevalence.However,its mechanisms are not clear.The present study was undertaken to illustrate the role of caveolin-1(cav1)and the scavenger receptor class B type 1(SR-B1)in NAFLD.METHODS:Adult male C57BL/6 mice were fed with a normal diet or high fat and cholesterol(HFC)diet for 14 weeks.The mice were sacrificed to collect plasma and harvest the liver;their plasma lipid concentration was measured.Hepatic cav1and SR-B1 mRNA and protein expression were determined by real-time quantitative polymerase chain reaction(qPCR)and Western blotting,respectively.In order to study cav1 and SR-B1distribution and change in hepatocytes,immunohistochemical analysis was performed.RESULTS:HFC diet increased plasma lipids,induced NAFLD and increased the liver/body weight ratio.Compared to the control mice(n=6),the mRNA and protein levels of cav1 and SR-B1 in liver tissue of the NAFLD mice(n=12)increased significantly(cav1 mRNA:1.536±0.226 vs 0.980±0.272,P<0.05;protein:0.643±0.240 vs 0.100±0.130,P<0.01;SR-B1 mRNA:1.377±0.125 vs 0.956±0.151,P<0.01;protein:2.156±0.507vs 0.211±0.211,P<0.01).Furthermore,both cav1 and SR-B1immunoreactivity increased and their distribution was also changed,mainly in the plasma membrane of hepatocytes,cytoplasm and membrane of lipid droplets and around.CONCLUSION:NAFLD is associated with increased concentration of plasma lipids and upregulation of hepatic cav1 and SR-B1 gene and protein expressions,which indicate that cav1 and SR-B1 might play crucial roles in the pathogenesis of NAFLD.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1499-3872DOI: 10.1016/S1499-3872(13)60099-5Titel-ID: cdi_proquest_miscellaneous_1467065281
- Format
- –
- Schlagworte
- and, Animals, Caveolin 1 - metabolism, caveolin-1, CD36 Antigens - metabolism, cholesterol, Cholesterol, Dietary - adverse effects, class, diet, Dietary Fats - adverse effects, disease, Disease Models, Animal, Endocrinology & Metabolism, fat, fatty, Fatty Liver - etiology, Fatty Liver - metabolism, Gastroenterology and Hepatology, high, high fat and cholesterol diet, liver, Liver - metabolism, Liver - pathology, Male, Mice, Mice, Inbred C57BL, non-alcoholic, Non-alcoholic Fatty Liver Disease, pathogenesis, receptor, RNA, Messenger - metabolism, scavenger, scavenger receptor class B type 1, type, Up-Regulation - physiology