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Highlights • Healthy subjects and patients suffering from mitochondrial myopathy underwent brachial artery occlusion. • Muscle (de)oxygenation (oxy[Hb+Mb] and deoxy[Hb+Mb] was registered with NIRS during and following occlusion. • The kinetics of deoxy[Hb+Mb] were slower in patients during occlusion. • The kinetics of oxy[Hb+Mb] were faster compared to deoxy[Hb+Mb] in both groups, resulting in a transient increase in total[Hb+Mb]. • The kinetics of both oxy[Hb+Mb] and deoxy[Hb+Mb] were faster in patients compared to healthy subject post occlusion.