Details

Autor(en) / Beteiligte

• Heberle-Bors, E
• Charvat, B
• Thompson, D
• Schernthaner, J.P
• Barta, A
• Matzke, A.J.M
• Matzke, M.A

Titel

Genetic analysis of T-DNA insertions into the tobacco genome

Ist Teil von

• Plant cell reports, 1988-12, Vol.7 (7), p.571-574

Ort / Verlag

Berlin: Springer

Erscheinungsjahr

1988

Quelle

SpringerLINK Contemporary (Konsortium Baden-Württemberg)

Beschreibungen/Notizen

• A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedlings were scored. Nine categories of transformants could be distinguished according to the number of loci into which T-DNA had inserted, and according to the effects of T-DNA integration on seed or seedling development. In most of the plants, T-DNA was inserted into a single site; others contained multiple independent copies of T-DNA. The number of T-DNA integration sites was found to be independent of whether a binary vector system or a cointegrate Ti plasmid had been used to obtain the transgenic plant. Loss of marker genes or marker gene expression from generation to generation appeared to be a quite frequent event. Plants which appeared to be insertional recessive embryo-lethal mutants did not exhibit this trait in the next generation.