Details

Autor(en) / Beteiligte

• Shapyrina, E. V
• Shadrin, A. M
• Solonin, A. S

Titel

Purification of recombinant Bacillus cereus ResD-ResE proteins expressed in Escherichia coli strains

Ist Teil von

• Applied biochemistry and microbiology, 2013-11, Vol.49 (6), p.542-548

Ort / Verlag

Boston: Springer-Verlag

Erscheinungsjahr

2013

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Recombinant E. coli strains expressing the Bacillus cereus ATCC 14579ᵀ resD and resE genes fused with the ubiquitin gene were constructed, and purification of the ResD and ResE proteins was performed. The approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous ResD and ResE proteins without denaturation steps up to 150 mg per gram of wet cell weight.