Proceedings of the National Academy of Sciences - PNAS, 2013-11, Vol.110 (46), p.18501-18506
2013
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Details
- Autor(en) / Beteiligte
- Titel
- Cytosolic reducing power preserves glutamate in retina
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2013-11, Vol.110 (46), p.18501-18506
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Glutamate in neurons is an important excitatory neurotransmitter, but it also is a key metabolite. We investigated how glutamate in a neural tissue is protected from catabolism. Flux analysis using ¹³C-labeled fuels revealed that retinas use activities of the malate aspartate shuttle to protect >98% of their glutamate from oxidation in mitochondria. Isolation of glutamate from the oxidative pathway relies on cytosolic NADH/NAD ⁺, which is influenced by extracellular glucose, lactate, and pyruvate.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.1311193110Titel-ID: cdi_proquest_miscellaneous_1458502746
- Format
- –
- Schlagworte
- Analysis of Variance, Animals, Biological Sciences, Carbon Isotopes - metabolism, Cell lines, Cellular metabolism, Cytosol - metabolism, Fuels, Gas Chromatography-Mass Spectrometry, Glutamic Acid - metabolism, HeLa Cells, Humans, Lactates, Metabolic Flux Analysis, Metabolites, Mice, Mice, Inbred C57BL, Mitochondria, Models, Biological, Neurons, Neurotransmitters, Oxidation, Oxidation-Reduction, Photoreceptors, Protein metabolism, Retina, Retina - metabolism, Tissues, Tricarboxylic acid cycle