Details

Autor(en) / Beteiligte

• Wojnicz, Aneta
• Cabaleiro-Ocampo, Teresa
• Román-Martínez, Manuel
• Ochoa-Mazarro, Dolores
• Abad-Santos, Francisco
• Ruiz-Nuño, Ana

Titel

A simple assay for the simultaneous determination of human plasma albendazole and albendazole sulfoxide levels by high performance liquid chromatography in tandem mass spectrometry with solid-phase extraction

Ist Teil von

• Clinica chimica acta, 2013-11, Vol.426, p.58-63

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• A simple, reproducible and fast (4min chromatogram) method of liquid chromatography in tandem with mass spectrometry (LC/MS–MS) was developed to determine simultaneously the plasma levels of albendazole (ABZ) and its metabolite albendazole sulfoxide (ABZOX) for pharmacokinetic and clinical analysis. Each plasma sample was extracted by solid phase extraction (SPE) using phenacetin as internal standard (IS). The extracted sample was eluted with a Zorbax XDB-CN column using an isocratic method. The mobile phase consisting of water with 1% acetic acid (40%, A) and MeOH (60%, B), was used at a flow rate of 1mL/min. ABZ and ABZOX were detected and identified by mass spectrometry with electrospray ionization (ESI) in the positive ion and multiple-reaction monitoring (MRM) mode. The method was linear in the range of 5–1000ng/mL for ABZ and 10–1500ng/mL (full validation) or 10–5000ng/mL (partial validation) for ABZOX, with 5 and 10ng/mL lower limit of quantification (LLOQ) for ABZ and ABZOX, respectively. The tests of accuracy and precision, matrix effect, extraction recovery and stability of the samples for both ABZ and ABZOX did not deviate more than 20% for the LLOQ and no more than 15% for other quality controls (QCs), according to regulatory agencies. [Display omitted] •Liquid chromatography with mass spectrometry to monitor antihelmintic plasma levels•Solid phase extraction as sample preparation under isocratic conditions•Method validated according to the recommendations of the regulatory agencies•Simple and fast method of 4min of recording time to improve patient's treatment