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This study documents variation of stable-carbon isotope ratios (^1^3C/^1^2C) in trees of genera Juniperus and Pinus under field conditions. Results are from cellulose analysis on leaves, twigs, and wood from a number of localities in the southwestern United States. Substantial variability, typically 1-3@%, exists among leaves, within wood (radially, vertically, circumferentially), and between individuals at a site. These results may help guide sampling in tracer-type studies with stable-carbon isotope ratios and aid in the interpretation of isotopic results from such studies.