Details

Autor(en) / Beteiligte

• MOORE, G. A
• JACONO, C. C
• NEIDIGH, J. L
• LAWRENCE, S. D
• CLINE, K

Titel

Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants

Ist Teil von

• Plant cell reports, 1992-06, Vol.11 (5-6), p.238-242

Ort / Verlag

Berlin: Springer

Erscheinungsjahr

1992

Quelle

SpringerLINK Contemporary (Konsortium Baden-Württemberg)

Beschreibungen/Notizen

• A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS(+). Polymerase chain reaction (PCR) analysis confirmed that GUS(+) shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.