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A method of actuator fault detection and isolation for a class of Lipschitz nonlinear systems with unknown disturbance is proposed based on reduced-order observers. Through equivalent state transformation, the state equation of the system is partitioned into two parts. Reduced-order observer was designed for the part de-coupled from unknown disturbance. The reduced-order observer is able to track the system state asymptotically when no actuator fault occurs. And the residual was designed to detect the actuator fault when an actuator is in faulty condition. In order to isolate the faulty actuator, the multi-observer strategy was adopted. The simulation results to a simplified dynamics of the high incidence research model (HIRM) aircraft show that the proposed method is effective.