International journal of cancer, 2012-09, Vol.131 (5), p.1131-1141
2012
Details
- Autor(en) / Beteiligte
- Titel
- Neuroblastoma triggers an immunoevasive program involving galectin-1-dependent modulation of T cell and dendritic cell compartments
- Ist Teil von
- International journal of cancer, 2012-09, Vol.131 (5), p.1131-1141
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- The immunosuppressive strategies devised by neuroblastoma (NB), the most common solid extracranial childhood cancer, are poorly understood. Here, we identified an immunoevasive program triggered by NB through secretion of galectin‐1 (Gal‐1), a multifunctional glycan‐binding protein. Human and mouse NB cells express and secrete Gal‐1, which negatively regulates T cell and dendritic cell function. When injected subcutaneously in syngeneic A/J mice, knockdown transfectants expressing low amounts of Gal‐1 (NXS2/L) showed reduction of primary tumor growth by 83–90% and prevented spontaneous liver metastases in contrast to NXS2 cell variants (NXS2/H, NXS2 wildtype) expressing high amounts of Gal‐1. Splenocytes from mice receiving Gal‐1 knockdown NXS2/L cells secreted higher amounts of IFN‐γ and displayed enhanced cytotoxic T‐cell function compared to NXS2/H or NXS2 controls. Immunohistochemical analysis revealed a six‐ to tenfold increase in the frequency of CD4+ and CD8+ T cells infiltrating tumors from mice receiving knockdown transfectants. This effect was confirmed by in vitro migration assays. Finally, supernatants of NXS2/H or NXS2 cells suppressed dendritic cell (DC) maturation and induce T cell apoptosis, whereas these effects were only marginal on DCs and T cells exposed to supernatants from NXS2/L cells. These results demonstrate a novel immunoinhibitory role of the Gal‐1‐glycan axis in NB, highlighting an alternative target for novel immunotherapeutic modalities.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0020-7136eISSN: 1097-0215DOI: 10.1002/ijc.26498Titel-ID: cdi_proquest_miscellaneous_1448214221
- Format
- –
- Schlagworte
- Animals, Antigen-Presenting Cells - immunology, Antigen-Presenting Cells - metabolism, Antigen-Presenting Cells - pathology, Apoptosis, Biological and medical sciences, Blotting, Western, Cancer, CD4-Positive T-Lymphocytes - immunology, CD4-Positive T-Lymphocytes - metabolism, CD4-Positive T-Lymphocytes - pathology, CD8-Positive T-Lymphocytes - immunology, CD8-Positive T-Lymphocytes - metabolism, CD8-Positive T-Lymphocytes - pathology, Cell Adhesion, Cell Movement, Cell Proliferation, dendritic cells, Dendritic Cells - immunology, Dendritic Cells - metabolism, Dendritic Cells - pathology, Female, Flow Cytometry, Galectin 1 - genetics, Galectin 1 - metabolism, galectin-1, Genetic Therapy, Humans, Immunoenzyme Techniques, Immunotherapy, Interferon-gamma - metabolism, Lung Neoplasms - immunology, Lung Neoplasms - metabolism, Lung Neoplasms - prevention & control, Lung Neoplasms - secondary, Lymphocytes, Medical research, Medical sciences, Mice, Mice, Inbred A, neuroblastoma, Neuroblastoma - immunology, Neuroblastoma - metabolism, Neuroblastoma - pathology, Neuroblastoma - prevention & control, Neurology, Rodents, T cell receptors, T cells, T-Lymphocytes, Cytotoxic - immunology, T-Lymphocytes, Cytotoxic - metabolism, T-Lymphocytes, Cytotoxic - pathology, tolerance, Tumor Cells, Cultured, Tumors, Tumors of the nervous system. Phacomatoses