Details

Autor(en) / Beteiligte

• Huits, H.S.M
• Wijsman, H.J.W
• Koes, R.E
• Gerats, A.G.M. (Vrije Univ., Amsterdam (Netherlands). BioCentrum. Inst. for Molecular Biological Sciences. Dept. of Genetics)

Titel

Genetic characterisation of Act1, the activator of a non-autonomous transposable element from Petunia hybrida

Ist Teil von

• Theoretical and applied genetics, 1995-07, Vol.91 (1), p.110-117

Ort / Verlag

Heidelberg: Springer

Erscheinungsjahr

1995

Quelle

SpringerLink_现刊

Beschreibungen/Notizen

• The line W138 of Petunia hybrida has variegated flowers because it is homozygous for the mutable an 1-W138 allele. Excision of the element, causing instability, depends on the presence of the activator Act1. The previously characterised non-autonomous element dTph1 excises from the dfrC gene in response to Act1. This implies that both non-autonomous elements belong to the same transposable element family. In a range of distantly related cultivars a single functional Act1 element could be detected. Linkage analysis for 11 of these lines showed that Act1 was located on chromosome 1 in all cases, indicating that the element might be fixed in the genome. A group of cultivars that did not exhibit Act1 activity could be traced back to a recent common origin ('Rose of Heaven'). Cultivars within this group presumably harbour the same inactivated Act1 element. Among the lines tested were 7 lines representing the two species (Petunia axillaris and Petunia integrifolia) from which P. hybrida originated. None of these exhibited Act1 activity. It is assumed that Act1 is present in an inactive state in these lines and that it was activated upon interspecific crossing. In general, lines representing the two parental species and P. hybrida cultivars contain between 5 and 25 dTph1 elements. The lines R27 and W138, however, contain significantly more dTph1 elements (over 50) than all other lines.