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Quantitative X-ray microanalysis of calcium in degenerating muscles: Preliminary results
Ist Teil von
Biology of the cell, 1985-01, Vol.54 (3), p.15a-15a
Erscheinungsjahr
1985
Link zum Volltext
Beschreibungen/Notizen
A quantitative X-ray microanalysis study of cell calcium compartments was carried out during the first 22 hr of the spontaneous breakdown in metamorphosing muscles of insects (Antheraea ). Freeze fixations without cryoprotectants followed by freeze substitution in the presence of oxalic acid are probably convenient methods of preparation for the analysis of calcium ions in cell compartments. Calcium peak-to-background ratios were determined in ultrathin sections of freeze-substituted specimens over four compartments (the electron-dense dorplets (EDDs) of the T system, the mitochondria, the myoplasm with myofilaments and dyads) using a wavelength dispersive crystal spectrometer (WDS). Calcium peak-to-continuum ratios obtained over the EDDS are significantly higher than in cell organelles, except in fibers containing calcium precipitates inside mitochondria.