Details

Autor(en) / Beteiligte

• Lage, OM
• Vasconcelos, MTSD
• Soares, HMVM
• Osswald, J M
• Sansonetty, F
• Parente, A M
• Salema, R

Titel

Suitability of the pH Buffers 3-[N-N-bis(hydroxyethyl)amino]-2-hydroxypropanesul- fonic Acid and N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic Acid for in Vitro Copper Toxicity Studies

Ist Teil von

• Archives of environmental contamination and toxicology, 1996-08, Vol.31 (2), p.199-199

Erscheinungsjahr

1996

Quelle

SpringerLINK Contemporary (Konsortium Baden-Württemberg)

Beschreibungen/Notizen

• In vitro copper toxicity studies were conducted using the marine dinoflagellate, Amphidinium carterae, to examine the impact of two pH buffers-3-[N-N-bis(hydroxyethyl)amino]-2-hydrox- ypropanesulfon- ic acid (DIPSO) and N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)-on Cu toxicity. The experimental protocol is detailed. Results indicated that DIPSO was a strong Cu-complexing agent, which was not observed for HEPES. Neither buffer, at DIPSO and HEPES concentrations of 10 and 25 mM, respectively, was toxic to A. carterae. However, HEPES did not complex bivalent Cu significantly, which increased the toxicity of the metal, possibly due to changes in the kinetics of metal binding to the organism.