Details

Autor(en) / Beteiligte

• Ouellet, Justine
• Berthiaume, Maryse
• Corriveau, Stéphanie
• Rola-Pleszczynski, Marek
• Pasquier, Jean-Charles

Titel

Effect of interleukin-6 receptor blockade on feto-maternal outcomes in a rat model of intrauterine inflammation

Ist Teil von

• The journal of obstetrics and gynaecology research, 2013-10, Vol.39 (10), p.1456-1464

Ort / Verlag

Australia: Blackwell Publishing Ltd

Erscheinungsjahr

2013

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Aim To study the effect of blocking the inflammatory cascade with interleukin‐6 receptor antibody (anti‐IL‐6R) on feto‐maternal outcomes in a rat model. Methods Pregnant Sprague–Dawley rats (n = 38) were injected intraperitoneally (day 22) (control, anti‐IL‐6R 30 μg/kg, lipopolysaccharide [LPS] 250 μg/kg or 500 μg/kg alone or combined with anti‐IL‐6R) followed by preterm caesarian performed 12 h later. Resuscitated pups (n = 179) were given to surrogate mothers. Primary outcomes were maternal and pup mortality. Results Fifty percent of pregnant rats died after LPS 500 μg/kg + anti‐IL‐6R injection but none in other groups. Neonatal mortality at 24 h was 63% and 86% in LPS 500 μg/kg and LPS 500 μg/kg + anti‐IL‐6R groups, respectively (P < 0.05). Surviving pups in the latter group presented a severe growth deficit compared to the LPS 500 μg/kg group (P < 0.01) and showed no difference with controls for open field testing. Maternal cytokine analysis after LPS 500 μg/kg + anti‐IL‐6R injection showed a tendency for increased IL‐1 production (P = 0.06). Conclusion Paradoxically, the association of pregnancy, inflammation and anti‐IL‐6R increases the inflammatory effects of LPS.